Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Bacoside-A exerts protective effect against Parkinson’s disease-induced functional damage in mice via inhibition of apoptosis and oxidative response

Binbin Zhang^1,2, Jiankuan Shi³, Lei Chang⁴, Hong Wang⁵, Yaping Wang⁶, Minxia Li³, Yuying Li¹ Yijun Song¹

¹Department of Neurology, Tianjin Medical University General Hospital, Tianjin 300052; ²Department of Neurology, Dongli Hospital, Dongli District, Tianjin 300300; ³Department of Neurology, Xi'an International Medical Center Hospital, Xi'an, Shaanxi 710100; ⁴Department of Neurology, The Third Hospital of Weinan City, Weinan, Shaanxi 714100; ⁵Department of Neurology, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120; ⁶Department of No.3 Cardiology, Shanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, China.

For correspondence:- Yijun Song Email: songyijun2000@126.com

Accepted: 10 November 2020 Published: 30 December 2020

Citation: Zhang B, Shi J, Chang L, Wang H, Wang Y, Li M, et al. Bacoside-A exerts protective effect against Parkinson’s disease-induced functional damage in mice via inhibition of apoptosis and oxidative response. Trop J Pharm Res 2020; 19(12):2565-2570 doi: 10.4314/tjpr.v19i12.12

© 2020 The authors.
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Abstract

Purpose: To determine the effect of bacoside-A on Parkinson's disease (PD) in a rat model, and elucidate its mechanism of action.

Methods: A rat model of PD was established by administration of 5 µL of 6-hydroxydopamine in ascorbic acid (0.1 %). Measurement of serum levels of inflammatory factors was carried out using enzyme-linked immunosorbent assay (ELISA) kits. Western blotting was used to assay Bax, cytochrome c and Bcl-2 in rat hippocampus.

Results: Bacoside-A treatment significantly reduced PD-induced high turning values in rats (p < 0.05). Treatment with bacoside-A reversed PD-mediated suppression of serum activities of CAT and glutathione peroxidase (GPx). In bacoside-A-treated PD rats, dose-dependent suppression of acetylcholinesterase (AChE) and inducible nitric oxide synthase (iNOS) activities were observed (p < 0.05). Bacoside-A-treated PD rats significantly (p < 0.018) reduced interleukin (IL)-1β and IL-6 levels. Treatment of PD rats with bacoside-A effectively reduced levels of tumor necrosis factor (TNF)-α, NF-κB p65, (COX)-2 and p53 protein, and also reversed up-regulations of Bax, cytochrome C, caspase-3 and caspase-9.

Conclusion: Bacoside-A exhibits a protective effect against Parkinson disease-induced oxidative damage and neuronal degeneration in rats through downregulation of iNOS, AChE, inflammatory cytokines and pro-apoptotic proteins. Therefore, bacoside-A has potentials for use in the management of Parkinson disease.

Keywords: Parkinson disease, Neuroprotective, Pro-apoptotic, Cytokines, Neurotoxicity